Journal: mAbs
Article Title: Bifunctional molecules targeting SARS-CoV-2 spike and the polymeric Ig receptor display neutralization activity and mucosal enrichment
doi: 10.1080/19420862.2021.1987180
Figure Lengend Snippet: Transcytosis in lung microtissues . A) Transcytosis of bifunctional molecules in EpiAirway tissue model in 24 h post application. For each sample, 20 mg of protein was added to the basolateral well, and after 24 hr, the mucosal surface was washed and the levels of transcytosed antibodies were quantified. Levels are shown in total micrograms transcytosed in a 24 h period. Error bars represent standard error and are representative of at least 2 independent experiments. B-C) Confocal image showing the human EpiAirway microtissue for CV19B307 (b) and CV19B290 (c). Staining shows: blue (nuclei), green (anti-VHH), and red (pIgR). Scale bars shows 50 mm (insets) and 100 mm (main image)
Article Snippet: Primary antibodies against pIgR (R&D Systems, MAB27171) and VHH domains (Genscript, A01995) were diluted to 5 mg/mL final concentration in PBST supplemented with 10% FBS and 500 mL was applied to the apical and basolateral chamber for 2 h at room temperature.
Techniques: Staining